जर्नल ऑफ क्लिनिकल एंड एक्सपेरिमेंटल फार्माकोलॉजी

अमूर्त

Ultrastructural Nuclear Changes in Mice Spleen Lymphocytes after Vanadium Inhalation

Rodr?guez-Lara Vianey ,Aleman-Muench Germán ,Soldevila Gloria ,García-Zepeda Eduardo ,Fortoul Teresa I *

Vanadium (V) is an air pollutant, which results from burning of fossil fuels, liberated as vanadium oxides, which are the most toxic compounds. Spleen is a vanadium target organ, and scarce research of the effects of vanadium and the immune system has been published. We previously report histologic modifications in the spleen in a mice inhalation model characterized by a very large and non-clearly delimited germinal centers size, as well as an increased CD19+ cells and a decline in antibody production after immunization. Since nuclear integrity is the support for genetic expression and cell cycle control, we decided to explore, in our model ultrastructural nuclear changes. Male mice were exposed to V205 (0.02M) for three months, twice a week by inhalation Spleen was obtained and processed for TEM. We observed striking nuclear changes such as: invaginations, blebs, multilobulations, and chromatin redistribution, pseudoinclusions, increased nucleolar size and number. All these changes suggest a direct interaction of vanadium with nucleoskeleton as it has been reported for its cytoplasmic counterpart. These alterations in nuclear structure could endorse neoplastic changes, which will need further evaluation.