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Simultaneous HPLC Analysis of Betamethasone and Clotrimazole in Cream Formulations

Adnan Manassra, Mustafa Khamis, Magdy el-Dakiky, Zuhair Abdel-Qader and Fuad Al-Rimawi

An HPLC method for the simultaneous quantitative determination of betamethasone and clotrimazole in cream formulation has been developed. The method utilizes a reversed-phase C18 (250 X 4.0 mm) stationary phase, with a mixture of methanol-acetate buffer-acetonitrile (33:27:40, v/v) as a mobile phase, and spectrophotometric UV detection at 254 nm. The method has been validated for cream formulations containing betamethasone and clotrimazole with linear range of 0.025 to 0.075 mg/ml for betamethasone with a correlation coefficient of 0.9996, and linear range of 0.25 to 0.75 mg/ml for clotrimazole with a correlation coefficient of 1.000. The results demonstrated that this method is accurate, precise, specific, linear, reliable, sensitive, and fast.