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Remarks on an Enzymatic Amplification System for Use in Point of Care Diagnostics

Marcia L Moss

Detection methods to quantify specific protein levels in biological samples for diagnostic purposes typically rely on the use of enzyme linked immunosorbent assays (ELISA). With an ELISA, total protein, rather than the bioactive amount may be quantified, as it is often complexed to naturally occurring aggregates or inhibitors. Thus, in cases where the protein is an enzyme, an activity assay may be more informative and correlative with disease status.