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Genetic Diversity Analysis in Chickpea Employing ISSR Markers

Gautam AK, Gupta N, Bhadkariya R, Srivastava N and Bhagyawant SS*

In the present study, inter simple sequence repeat (ISSR) marker were employed to estimate genetic diversity in 13 accessions of chickpea including cultivated and wild. Among all these anchored ISSR primers tested, pentanucleotide repeat primer UBC-879 produced better amplification patterns. A total of 150 bands were amplified in a molecular weight range of 100-2000 bps revealing an average of 21.4 bands per primers and 1.64 bands per primer per genotype. The repeats (GA)₈C, (AG)₈YT, (GA)₈YC, (AG)₈C, (GTT)₆ and (GT)₈YC give least amplification. UPGMA dendrogram constructed between these accessions depicted three major clusters. Based on genetic origin and diversity index viz. ICC-14051, ICC-13441, ICC-15518, ICC-12537, and ICC-17121 may be recommended to be selected as a parent in future breeding programmer for chickpea.