एक्वाकल्चर अनुसंधान एवं विकास जर्नल

अमूर्त

Comparative Analysis of Genetic Diversity in Natural Population of Labeo rohita and Cirrhinus marigala by Using Species Specific Molecular Markers

Faisal Tasleem*, Shahid M, Hina Fatima, Numan Gulzar M

Labeo rohita, commonly known as rohu and Cirrhinus marigala, popularly known as morakkha are economically most important and extensively cultured fish species in the entire Indian subcontinent. Genetic evaluation of these species is necessary for their proper supervision conservation and profitable production. Various types of molecular markers are available but SSR are most important one due to their equal distribution, abundance in the genome, co- dominance, polymorphic, low cost detection and multi-allelic nature. In this study ten samples of each species were collected from Head Punjnad, Head Muhammad Wala and Head Trimmu region of river Chenab, Pakistan. After the extraction of DNA, amplification of five simple sequence repeat markers, their resolution on PAGE and allelic scoring for the genotypic data was used for the analysis of different genetic diversity indices with the help of POPGENE version 1.32, FSTAT version 2.9.3.2, Jaccard and Dice similarity coefficient, simple matching analysis and SIMQUAL program of NTSYS-PC package.

In case of Labeo rohita, total 26 alleles with 260 loci were detected out of which 189 were found polymorphic. Polymorphism for the five markers varies from 43.33%-96% with mean value of 72.69%. Allelic frequency ranges from 0.2000-0.9000 with 0.4600 mean value, allele numbers varies from 4.000-9.000 with average value of 5.2000, gene diversity varies from 0.1800-8800 with mean value of 0.6360 and PIC value ranges from 0.1638-0.8680 with 0.6012 mean value. Pair wise genetic difference among ten collected samples varies from 0.400-0.900 while pair wise genetic similarity ranges from 0.100-1.000. Cluster analysis based on UPGMA divided ten samples of Labeo rohita into three clusters and three sub clusters. Similarly, five SSR markers of Cirrhinus marigala show 30 alleles with 300 loci across ten samples with 240 polymorphic loci. Polymorphism ranges from 70%-96% with average value of 80%. Allelic frequency varies from 0.4000-0.9000 with average value of 0.6600. Allele numbers varies from 4.000-9.000 with 6.000 as mean value. Gene diversity varies from 0.1800-0.7800 with mean value of 0.4680. PIC value ranges from 0.1638-0.7578 with mean value of 0.4422. Pair wise genetic difference among ten samples of Cirrhinus marigala ranges from 0.2000-0.800 and pair wise similarity ranges from 0.200-1.000. Cluster analysis based on UPGMA divided all the samples of Cirrhinus marigala into three clusters and three sub clusters. This study reveals that Government of Pakistan and interested agencies should take serious steps for the improvement of genetic structure of these species especially Cirrhinus marigala.