Shahnaz Dilawar, Afzal Shah, Zahoor Ahmad
The serum samples were collected from patients and healthy subjects of same. Age and gender groups for comparative study. Serum samples were oxidized by 60% nitric, acid and 70% hypochloric acid” The serum selenium (Se) concentration was measured using HGAAS (hydride generation atomic absorption spectrophotometerHS-55 batch system). Mean ±SD of serum Se levels (µg/L) were 82 ±1.8 and 108 ±1.3 in patients and controls respectively. T-test and One-Way ANOVA were applied. This case control study was carried out in Bannu University of science and technology Pakistan with the collaboration of Govt. Sifwat Ghayur Shaheed memorial children hospital Peshawar Pakistan, NIH (National institute of medical sciences) and National Physical Standard Laboratory PCSIR Islamabad from April 2013 to April 2015.