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अमूर्त

HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples

Malik Alamgir, Muhammad Yar Khuhawar, Saima Qayoom Memon, Amir Hayat, Rizwan Ali Zounr and Asma Chanar

A novel High Performance Liquid Chromatography (HPLC) method has been developed based on pre column derivatization with benzoin for determination of metformin, famotidine and ranitidine. The separation was achieved from C18 column when eluted isocratically, the solution of the drugs with methanol, water, acetonitrile and Tetra Hydrofuran (THF) (40:40:16:4 v/v) with a flow rate at 1 mL/min. UV detection was found to be 268 nm. Linear calibrations range was obtained with 2.5-12.5 μg/ml, with limits of detection (LODs) 0.091-0.30 μg/ml. The total run time for elution was 3.5 min. The derivitization, separation and quantitation was repeatable (n=4) in terms of retention time and peak height/peak area with Relative Standard Deviation (RSD) within 0.84-1.55% and 0.68-1.17% respectively. The method was applied for the analysis of metformin, famotidine and ranitidine from pharmaceutical preparations, human serum and human urine. The possible interfering effects of the sample matrix were checked by the analysis by standard addition method and matrix effect was not indicated.